SnapShot: Mouse Primitive Streak
نویسندگان
چکیده
منابع مشابه
The in vitro culture of primitive-streak-stage mouse embryos.
The in vitro growth and morphogenesis of mouse embryos from early primitive-streak stage to early-somite stage is described. The embryo culture method employs a static culture system, a conventional chemically defined medium (Dulbecco's modified Eagle's medium), supplemented with additional glucose, glutamine and suitably prepared serum. The method of serum preparation is important for successf...
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A prospective fate map of the late gastrulation mouse primitive streak has been charted in 8.5 dpc mouse embryos developed in culture, using the lineage marker DiI to label groups of cells. As at earlier stages, the fate of cells in the 8.5 dpc primitive streak is regionalised such that successively more caudal regions of the streak give rise to more lateral mesoderm. While most labelled cells ...
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The strength and spatiotemporal activity of Nodal signaling is tightly controlled in early implantation mouse embryos, including by autoregulation and feedback loops, and involves secreted and intracellular antagonists. These control mechanisms, which are established at the extra-embryonic/embryonic interfaces, are essential for anterior-posterior patterning of the epiblast and correct position...
متن کاملChimaerism of primordial germ cells in the early postimplantation mouse embryo following microsurgical grafting of posterior primitive streak cells in vitro.
A microsurgical grafting technique has been used to introduce primordial germ cell (PGC) precursors into intact primitive-streak-stage mouse embryos in vitro. Operated embryos were cultured for 36-40 h and then analysed by a combined histochemical and autoradiographic method. PGC chimaerism occurred in embryos that received grafts of caudal primitive streak cells but not adjacent embryonic endo...
متن کاملTiming of X-chromosome inactivation in postimplantation mouse embryos.
The onset of X-chromosome inactivation was investigated cytologically in postimplantation female mouse embryos of age 5£, 6\ and 1\ days post-coitum (d.p.c.) and in the isolated epiblasts of 6 d.p.c. embryos before primitive streak formation using a heat/hypotonic technique to reveal the inactive X chromosome by differentially dark staining with Giemsa. The results indicate that X inactivation ...
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عنوان ژورنال:
- Cell
دوره 146 شماره
صفحات -
تاریخ انتشار 2011